The study on hepatocarcinoma cells cotransfected with B7.1 and SEA genes
10.3321/j.issn:1007-8738.2001.04.005
- VernacularTitle:B7.1及 SEA基因共转染肝癌细胞的初步研究
- Author:
Yumei ZHANG
;
Yanfang SUI
;
Quanjian YAN
;
Zengshan LI
;
Jun YU
- Publication Type:Journal Article
- From:
Journal of Cellular and Molecular Immunology
2001;17(4):313-314,340
- CountryChina
- Language:Chinese
-
Abstract:
Aim To obtain the HCC cell lines which could coexpressed the B7.1 and SEA. Methods The positive clones expressing the B7.1 and SEA were screened by immunohistochemical staining. The amount of SEA in culture supernatant was detected by ELISA. Results HCC cell clones coexpressing B7.1 and SEA were obtained, and expression amount of SEA in culture supernatant reached 10～ 14× 10-8g/L. Conclusion The co-rec-ogenition immune effective system of SEA and B7.1 on HCC cells is established.
