Downregulation of PGC1α expression in hypothalamus of rats caused by short-term high-fat diet

VernacularTitle:短期高脂饮食引起大鼠下丘脑PGC1α表达下调
Author: Li ZHONG ; Bangqiong WANG ; Qifu LI ; Qingfeng CHENG
Publication Type:Journal Article
Keywords: PGC1α; High-fat diet; Rats; Hypothalamus
From: Journal of Endocrine Surgery 2011;05(4):242-243,246
CountryChina
Language:Chinese
Abstract: ObjectiveTo explore the effects of short-term high-fat diet on expression of peroxisome proliferator-activated receptor-γ-coactivator 1α (PGC1α) in hypothalamus of rats. Methods18 male SD rats were randomly divided into 2 groups: the normal control group (NC) and the high-fat group (HF). After two-week adaptive feeding, the 2 groups were given control diet and high-fat diet respectively for 3 days. Body weight and energy intake were recorded every day. Random blood glucose of caudal vein was measured before and after intervention. The mRNA and protein levels of PGC1α, UCP2 and CPT1a in hypothalamus were detected by quantitative RT-PCR and Western Blot. ResultsAfter the three-day treatment, the energy intake in HF group was significantly increased compared with that in NC group (75.7 ±5. 02 vs 60.4 ±4.53 kcal/d, P ＜0.05). However,the body weight, food intake and blood glucose level were not significantly changed. The mRNA level of PGC1α was significantly decreased (P ＜0.05 ), while no significant difference was observed in protein levels. Conclusion Short-term high-fat diet downregu]ates the expression of PGC1α mRNA in hypothalamus of rats.