Clinical significance of microRNA-199b-5p expression in papillary thyroid carcinoma

VernacularTitle:微小RNA-199b-5p在甲状腺乳头状癌中的表达及意义
Author: You PENG ; Chen LI ; Dingcun LUO ; Jinwang DING ; Wo ZHANG ; Gang PAN
Publication Type:Journal Article
Keywords: Papillary thyroid carcinoma; microRNA; microRNA-199b-5p
From: Journal of Endocrine Surgery 2014;(4):268-271,281
CountryChina
Language:Chinese
Abstract: Objective To investigate the expression of miR-199b-5p in papillary thyroid carcinoma ( PTC) and its relationship with clinical features .Methods Total RNA was extracted from 36 cases of PTC and the adjacent normal thyroid tissues by reverse transcription quantitative real-time polymerase chain reaction ( qRT-PCR)method to detect the expression of miR-199b-5p, and to analyze its relationship with clinical features such as the capsule invasion and lymph node metastasis .Results miR-199b-5p expression in PTC was related to lymph node status(χ2 =9.20, P=0.01), capsule invasion(U=36.00, P=0.047), but had no correlation with other clinical characteristics such as age , sex, tumor size, the number of tumor foci ( U =151.00, 87.00, 64.00, 87.00 respectively, P>0.05).ROC curve analysis showed that the specificity and sensitivity of miR-199b-5p in diagnosis of PTC were 82.1% and 72.7% respectively.Conclusion The abnormal expression of miR-199b-5p may be related to the occurrence , development and invasion of PTC .