Anti-proliferative effect of artesunate on MDA-MB-231 cell and its mechanisms

VernacularTitle:青蒿琥酯对MDA-MB-231细胞增殖抑制作用及其机制
Author: Xiaobo ZHAO ; Tianyong XING ; Kainan WU
Publication Type:Journal Article
Keywords: Artesunate; MDA-MB-231 cell; Bax; nm23; P21WAF1/CIP1; Bcl-2
From: Journal of Endocrine Surgery 2011;05(3):155-157,175
CountryChina
Language:Chinese
Abstract: Objective To study the effects of artesunate(ART)on estrogen receptor negative breast cancer cell line MDA-MB-231 and its mechanisms.Methods Treated with ART for 3 days,MDA-MB-231 cells proliferation was examined by MTT assay.The morphological and uhrastructural changes of MDA-MB-231 cells were observed under microscope and electronic microscope.Immunocytochemistry was used to detect the expression of Bax,mn23,Bcl-2,and P21 WAFl/CIPl.Results ART treatment led to a dose dependent inhibition of MDA-MB-231 cells.ART could change the morphology and ultrastructure of MDA-MB-231 cens.After treatment with ART(2μmol/L)for 72 hours,immunocytochemical staining showed that the expression of Bax,nm23,and P21WAF1/CIP1 was upregulated in comparison to the control group(P<0.05)while the expression of Bcl-2 in MDA-MB-231 cells didn't have a significant change(P>0.05).Conclusions ART shows an anti-proliferative effect on MDA-MB-231 cells.The mechanisms may be related to upregnlation of Bax,nm23 and P21WAF1/CIP1 expression.