Saikosaponin D inhibits proliferation and induces apoptosis via C/EBPβ-p53 signal pathway in human hepatoma HepG2 cells
- Author:
Xinlan LU
;
Xi LIANG
;
Yaxin ZHANG
;
Yanan HU
;
Shuixiang HE
- Publication Type:Journal Article
- Keywords:
saikosaponin D;
p53;
CCAAT enhancer binding protein β;
apoptosis
- From:
Journal of Pharmaceutical Analysis
2010;22(4):252-254,259
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the anticancer effects and detailed mechanisms of Saikosaponin D (SSD) in human hepatoma HepG2 cells. Methods Cell proliferation and apoptosis were tested by MTT assay and Annexin-V/PI assay respectively. The expressions of CCAAT enhancer binding protein β(C/EBPβ) and p53 were detected by RT-PCR and Western blotting. Results SSD inhibited cell proliferation in a dose-dependent manner and induced apoptosis at the concentration of 5.0mg/L. SSD significantly increased the mRNA and protein levels of C/EBPβ and p53 in a dose-dependent manner. Conclusion SSD exerts its anticancer effect by inhibiting cell proliferation and inducing apoptosis partly through C/EBPβ-p53 signal pathway in HepG2 cells.
