The effect of granulocyte-macrophage-stimulating factor on the gene expression in human trophoblast of early pregnancy.
10.5468/kjog.2010.53.10.905
- Author:
Hun Yul LEE
1
;
Sang Soo KIM
;
Chang Suk AHN
;
Young Joo PARK
;
Jin Hyun JUN
;
Haengsuk SONG
;
Hye Sung MOON
;
Eun Joo PARK
;
Seo You HONG
;
Won Il PARK
Author Information
1. Department of Obstetrics and Gynecology, Eulji University School of Medicine, Daejeon, Korea. pwi3110@eulji.ac.kr
- Publication Type:Original Article
- Keywords:
Granulocyte-macrophage colony-stimulating factor;
Trophoblast;
Focal adhesion;
Invasion
- MeSH:
Actin Cytoskeleton;
Annexin A2;
Embryonic Development;
Female;
Focal Adhesions;
Gene Expression;
Granulocyte-Macrophage Colony-Stimulating Factor;
Humans;
Microarray Analysis;
Myogenin;
Pregnancy;
Trophoblasts;
Vimentin
- From:Korean Journal of Obstetrics and Gynecology
2010;53(10):905-914
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: Granulocyte-macrophage colony-stimulating factor (GM-CSF) facilitates mammalian embryonic development and implantation. However, its biological function after implantation is not elucidated. The aim of this study is to assess the changes of gene expression by GM-CSF in human trophoblast obtained in early pregnancy. METHODS: Human trophoblast obtained in early pregnancy was cultured with or without GM-CSF. The difference of gene expression was evaluated with microarray and selected genes were reevaluated with real-time reverse transcription-polymerase chain reaction (RT-PCR). RESULTS: Microarray analysis revealed that the expressions of 468 genes were increased while those of 40 genes were decreased by GM-CSF. These genes were evaluated according to the known biologic pathways. The regulation of actin cytoskeleton and focal adhesion pathways were mostly influenced by GM-CSF. Annexin A2, thymosin-like 3, vimentin, myogenin, ACK1, and tensin1 genes were selected for real-time RT-PCR. The increased expressions of of vimentin and ACK1, and decreased expressions of tensin1 were confirmed by real-time RT-PCR. CONCLUSION: GM-CSF activates focal adhesion pathway in human trophoblast by increasing the expression of vimentin and ACK1, and decreasing the expression of tensin1.
