Construction and identification of recombination expression vector Ksp-Cadherin-Gpx1-Kik1
- Author:
Liyi XIE
;
Wujun XUE
;
Heli XIANG
;
Sunkai MA
- Publication Type:Journal Article
- Keywords:
Gpx1;
Klk1;
Ksp-cadherin;
ischemic-reperfusion injury
- From:
Journal of Pharmaceutical Analysis
2008;20(4):217-220,255
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct and identify the Gpx1-Klk1 vector which contains kidney-specific promoter (Ksp-cadherin). Methods Through PCR amplification, the human Gpx1, Klk1, and Ksp-cadherin eDNA were obtained by taking Gpx1 cDNA, Klk1 eDNA, and Ksp-cadherin BAC as templates. After being testified, the PCR products were inserted into the expressive vector pIRES-EGFP step-by-step to produce a recombinant vector Ksp-cadherin-Gpx1-Klk1. This vector was examined by restriction enzyme digestion and sequence analysis. Results The recombinant expressive vector Ksp-cadherin-Gpx1-Klk1 was successfully constructed. Conclusion The construction of the recombinant vector Ksp-cadherin-Gpx1-Kik1 laid foundations for investigations in establishing transgenic animal models, the over-expression of Gpx1 and Kikl in mammal kidney, and gene therapy for ischemia-reperfnsion injury during kidney transplantation.
