EFFECT OF QUERCETIN ON CULTURED HUMAN VASCULARENDOTHELIAL CELLS
- Author:
Rong LIN
;
Juntian LIU
;
Xu LI
;
Wei CHEN
- Publication Type:Journal Article
- Keywords:
quercetin;
vascular endothelium;
cultured cells
- From:
Journal of Pharmaceutical Analysis
2000;12(1):17-18,封四
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of quercetin (Que) on the release of endothelin-1(ET-1) and prosta cylin(PGI2) by normal human vascular endothelial cell(VEC). Methods Radioimmunoassay(RIA) was used to assess the amount of ET-1 and PGI2 produced by VEC. VEC proliferation was assessed by tetrazolium(MTT) assay. Results Que increased the normal VEC proliferation at the concentration of 5, 20, 40, 80, 100μmol/L and increased the pro duction of PGI2 and inhibits the release of ET by the normal VEC at the concentration of 5, 20 and 80μmol/L. Que at the concentration of 5, 20 and 80μmol/L had no direct effect on morphology of the normal VEC. Conclusion Que can stimulate the proliferation of VEC and inhibit the release of ET-1 and increase the formation of PGI2. The data sug gest that Que might be beneficial for the prevention and treatment of vascular endothelial injury-related cardiovascu lar diseases, such as atherosclerosis and thromboembolism diseases.
