Detection of Mycoplasma Infection in Cultured Cells on the Basis of Molecular Profiling of Host Responses.
- Author:
Tae Su CHUNG
1
;
Ju Han KIM
;
Young Ju LEE
;
Woong Yang PARK
Author Information
1. Seoul National University Biomedical Informatics, Korea.
- Publication Type:Original Article
- Keywords:
Mycoplasma;
oligonucleotide microarray;
prediction model;
biomarker
- MeSH:
Cell Cycle;
Cells, Cultured*;
Gene Expression;
Mycoplasma Infections*;
Mycoplasma*;
Oligonucleotide Array Sequence Analysis;
Transcriptome
- From:Genomics & Informatics
2005;3(3):63-67
- CountryRepublic of Korea
- Language:English
-
Abstract:
Adaptive responses to diverse microbial pathogens might be limited in relatively few types. Host cell responses to pathogens are believed to be patterned or stereotyped along with species or class. We tried to compose the host response to Mycoplasma in terms of cellular gene expression. Although gene expression profile of two host HeLa and 293 cells were quite different each other, 30 genes were differentially expressed by mycoplasma infection in both of HeLa and 293 cells. Six of them (PR48, MADH4, MKPX, CRK, RBM7, NEK3) were related to cell cycle or proliferation. Another category of genes like IL1HY1, KLRF1, TNFSF14, GBP1 were host defense to elicit immune responses. With this set of genes, we establish the prediction model for mycoplasma contamination.
