The Role of GADD45beta in Acute Myocardial Injury.
- Author:
Sukki CHO
1
;
Jong Myeon HONG
;
Jeong Ryul LEE
;
Hak Mo LEE
;
Byong Chul OH
;
Jae Woong LEE
Author Information
1. Department of Thoracic and Cardiovascular Surgery, Kyungpook National University Hospital, College of Medicine, Kyungpook National University, Korea.
- Publication Type:Original Article
- Keywords:
Xenotransplantation;
GADD45beta;
Transfection;
Apoptosis;
H9C2
- MeSH:
Animals;
Apoptosis;
Cell Line;
Humans;
Myoblasts;
Myoblasts, Cardiac;
Rats;
Tissue Donors;
Transfection;
Transplantation, Heterologous;
Transplants
- From:The Korean Journal of Thoracic and Cardiovascular Surgery
2008;41(1):25-33
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: A critical shortage of donor organs has necessitated an investigation of new strategies to increase the availability of additional organs available for human transplantation. We investigated the amount of apoptosis and expression of GADD45beta in two groups, a GADD45beta-transfected group and untransfected group. MATERIAL AND METHOD: The experimental groups consist of a control group (normal H9C2 cell line) and GADD45beta-transfected group. After injury of the each group, we evaluated the expression of GADD45beta and the level of apoptosis in each group. RESULT: There was a significant increase in the expression of GADD45beta in the GADD45beta-transfected group at 1 hour, 2 hours, and 3 hours after stimuli as compared with the control group. The amount of cardiac myoblast cell line apoptosis was significantly lower in the GADD45beta-transfected group as compared with the control group. The concentration of annexin in the GADD45beta-transfected group was significantly lower than that of the control group after cell injury. CONCLUSION: Transfection of a rat myoblast cell line with the GADD45beta gene results in decreased susceptibility to cell injury of human serum.
