Co-cultivation of Adenovirus with Cultivated Conjunctival Epithelial Cell: Morphological Changes in Infected Cell.
- Author:
Myung Kyoo KO
1
;
Ki Tae KANG
;
Joon Kiu CHOE
Author Information
1. Department of Ophthalmology, School of Medicine Hanyang University, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Adenovirus;
Epidemic keratoconjunctivitis;
Inclusion body
- MeSH:
Adenoviridae*;
Crystallins;
Cytoplasm;
Epithelial Cells*;
Epithelium;
Humans;
Inclusion Bodies;
Keratoconjunctivitis;
Virion
- From:Journal of the Korean Ophthalmological Society
1993;34(7):606-612
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
This study was performed to investigate the morphological changes of the human conjunctival epithelium when the cultured conjunctival tissue was co-cultivated with the inoculated adenovirus. Specimens swabbed from the lower conjunctival sac of patients with epidemic Keratoconjunctivitis were inoculated into the cultured conjunctival epithelial cells and co-cultivated. The adenovirus-infected cell revealed viral particles, crystalline arrays of adenovirus, paracrystalline inclusions and three types of inclusions within the nucleus. The cytoplasm did not show the specific pathologic changes. These results suggest that adenovirus can be co-cultured with conjunctival epthelial cell and the infected cell showes the typical morphological changes within the nucleus. This in-vitro co-cultivation of adenovirus with conjunctival epithelial cell may be used for an in-vitro model in the research of adenovirus.
