Expression of Survivin gene in human breast cancer and its clinical significance

VernacularTitle:乳腺癌中Survivin基因的研究及其临床意义
Author: Kai LU ; Zhuangkai YAO ; Yanwen LIU ; Hongling YE ; Sanyun LV
Publication Type:Journal Article
Keywords: Breast cancer; Survivin; Lymph node metastasis; RT-PCR; Fluorescence in situ hybridization (FISH)
From: Journal of Endocrine Surgery 2012;06(5):323-326
CountryChina
Language:Chinese
Abstract: Ohjective To investigate the expression of Survivin gene in human breast cancer and its clinical significance.MethodsReal-time PCR technology and fluorescence in situ hybridization (FISH) technology were employed to detect the expression of Survivin gene in the following tissue samples:50 breast cancers in stage Ⅰ and Ⅱ,50 breast cancers in stage Ⅲ and Ⅳ,and the corresponding adjacent normal mammary tissues.The relationship of Survivin gene expression and several factors such as differentiation degree,invasion,lymph node metastasis,TNM stage was explored.ResultsThe positive expression rate of Survivin gene was 62％ and 78％ respectively detected by real-time PCR and FISH in breast cancer tissues.The overexpression of Survivin gene was positively correlated with TNM stage and lymph node metastasis.ConclusionsOverexpression of Survivin gene can be used as one of the parameters in early diagnosis and prognosis of human breast cancer.Selective gene therapy based on these gene expressions may be useful.