Study on bridged avidin biotin technique improving the sensitivity of phycobiliprotein immunofluorescence assay

VernacularTitle:桥式生物素－亲合素标记提高藻胆蛋白免疫荧光法灵敏度的研究
Author: Ping WU ; Ming GU ; Yihua QI ; Fan OUYANG
Publication Type:Journal Article
From: Journal of Cellular and Molecular Immunology 2001;17(4):321-323
CountryChina
Language:Chinese
Abstract: Aim To improve the sensitivity of phycobiliprotein immunofluorescence assay(PBPIFA), by introducing into bridged avidin biotin(BRAB) technique,known as BRAB-PBPIFA. Methods Using ELISA as a gold standard, effectiveness of three assays(direct PBPIFA, BRAB-PBPIFA and ELISA)were compared in simultaneous detection of standard antigen and 500 serum samples. Results BRAB improved greatly sensitivity of PBPIFA in detecting antigen of low concentration, and detection rate of weakly positive samples could increase by one times and a half. In the applying, protein-free blocking agent Tween 20 to BRAB-PBPIFA,possessed better blocking effect. Conclusion Combination of BRAB technique with PBPIFA can improve the detcctive sensitivity.