Study on S100A4 gene silence by siRNA and its inhibition on invasion of human thyroid carcinoma cell

VernacularTitle:siRNA沉默S100A4基因表达抑制甲状腺癌细胞侵袭的研究
Author: Pengcheng JIANG ; Yu FAN ; Yongjing ZHOU
Publication Type:Journal Article
Keywords: Thyroid carcinoma; siRNA; S100A4; Invasion; Matrix metalloproteinase
From: Journal of Endocrine Surgery 2010;04(6):371-374
CountryChina
Language:Chinese
Abstract: Objective To explore the effects and mechanism of S100A4 gene silence on invasion of human thyroid cancer cell. Methods After thyroid cancer cell ARO was transfected by S100A4 small interfering RNA (siRNA), mRNA and protein level of S100A4 and matrix metalloproteinase 2 (MMP-2) were determined by real time RT-PCR and Western blot respectively. The anchorage-independent growth was examined by colony formation assay in soft agar, and invasion ability was evaluated by boyden chamber model. Results The level of mRNA and protein of S100A4 was significantly inhibited in ARO cancer cells transfected by S100A4 siRNA.Transfection with S100A4 siRNA could inhibit anchorage-independent growth and invasion ability of thyroid cancer cell ARO in a dose-dependent manner. mRNA and protein expression of MMP-2 were down-regulated by S100A4 siRNA. Conclusion S100A4 siRNA can inhibit the invasion of thyroid cancer cell through down-regulation of MMP-2.