The effects of targeted forkhead box E1 gene interference on epithelial-mesenchymal transition in thyroid cancer cells
10.3760/cma.j.issn.1674-6090.2014.02.017
- VernacularTitle:靶向干扰叉头框 E1基因对甲状腺癌细胞上皮间质转化的影响
- Author:
Zheng DING
;
Youben FAN
;
Xianzhao DENG
;
Jie KANG
;
Bo WU
- Publication Type:Journal Article
- Keywords:
Thyroid cancer;
Forkhead box E1 gene;
Epithelial-mesenchymal transition;
Invasion and metastasis
- From:
Journal of Endocrine Surgery
2014;(2):141-144
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of targeted forkhead box E1( FOXE1) gene on epitheli-al-mesenchymal transition(EMT) of papillary thyroid cancer (PTC) cell line TPC-1, and to study its role in in-vasion and migration of TPC-1 cells.Methods The lentiviral expression vector for RNA interference of FOXE 1 gene was constructed to silence the expression of FOXE 1.Real-time polymerase chain reaction ( RT-PCR) and Western blot were used to detect the expression of such EMT markers as E-cadherin , N-cadherin and Vimentin . Transwell assay and Scratch assay were used to analyze TPC-1 cells migration and invasion .Results After RNA interference of FOXE1, the morphology of TPC-1 cells indicated a transformation of EMT .The expression of epi-thelial phenotype marker E-cadherin decreased remarkably while mesenchymal marker Vimentin was significantly up-regulated(P<0.01).Compared with the control group , the expression of Vimentin mRNA in the experimen-tal group was 2.24 times higher .The migration and invasion ability of TPC-1 cells increased significantly , and the number of cells in transwell was 2.11 times of the control's.Conclusion The silence of FOXE1 gene probably increases the invasion potential of human PTC cells through EMT .
