The apoptosis of mouse macrophage J774A. 1 induced by oxysterol depend on NF-κB activation
10.3321/j.issn:1007-8738.2009.10.005
- VernacularTitle:氧固醇诱导巨噬细胞J774A.1的凋亡依赖于NF-κB的活化
- Author:
Zhenyu HUANG
;
Qingping LIU
;
Wenzhe LI
;
Renjun WANG
;
Yan CHI
;
Yuanyuan LIU
;
Qiming ZHANG
- Publication Type:Journal Article
- Keywords:
7-KC;
NF-κB;
cell apoptosis;
macrophage
- From:
Journal of Cellular and Molecular Immunology
2009;25(10):879-882
- CountryChina
- Language:Chinese
-
Abstract:
AIM: Investigated the relationship between NF-κB activation and cell apoptosis in mouse macrophages treated with 7-ketochesterol (7-KC). METHODS: Cell apoptosis was detected by MTT assay, DNA fragmentation assay and flow cytometric analysis. NF-κB activation was detected by western blot and immunohistochemistry. Inhibitory assay was used to show the effect of the activation of NF-κB on the apoptosis induced by 7-KC. RESULTS: 7-KC inhibited macrophages proliferation, and then induced apoptosis, which is associated with NF-κB activation. Moreover, cell apoptosis with NF-κB activation was inhibited by pyrrolidine dithiocarbamate (PDTC), an inhibitor of NF-κB. CONCLUSION: 7-KC induced the activation of NF-κB and following cell apoptosis.
