Expression of PTD-bcr/abl fusion oncoprotein fragment carrying protein transduction domain and its transmembrane transportation
10.3321/j.issn:1007-8738.2001.04.028
- VernacularTitle:PTD-bcr/abl融合癌蛋白片段的表达及其跨膜转运
- Author:
Yingmin LIANG
;
Qiang SUN
;
Shanshan JIANG
;
Ping CHENG
;
Jishu WANG
;
Li LIU
;
Hua HAN
- Publication Type:Journal Article
- From:
Journal of Cellular and Molecular Immunology
2001;17(4):377-380
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the method of transfering oncoprotein bcr/abl, mediated by protein transduction domain (PTD), in order to provide the experimental basis for immune therapy. Methods DNA fragment encoding PTD was synthesized and fused with PCR-amplified bcr/abl gene fragment. The fusion protein was expressed in E.coli as His-tagged protein. The purified fusion protein was loaded to cultured HL-60 cells. Whether the fusion protein entered into HL-60 cells was determined by Western blot. Results It is shown that PTD could mediate transportation of bcr/abl protein to enter into cells. Conclusion These results may provide a new approach for loading exogenous proteins to antigen presenting cells.
