Preparation and identification of polyclonal antibody against protein H1b: the variant of major subunit of human ASGPR
10.3321/j.issn:1007-8738.2009.10.016
- VernacularTitle:抗人ASGPR大亚基异构体蛋白H1b多克隆抗体的制备和鉴定
- Author:
Jia LIU
;
Honghui DING
;
Yan YANG
;
Bin HU
;
Yuan YU
;
Hongping HUANG
;
Mengji LU
;
Dongliang YANG
- Publication Type:Journal Article
- Keywords:
ASGPR;
polyclonal antibody;
splice variant
- From:
Journal of Cellular and Molecular Immunology
2009;25(10):917-919
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To prepare and identify mouse polyclonal antibody against protein Hlb, which is the variant of major subunit of human ASGPR. METHODS: Hlb specific peptide was synthesized and coupled with keyhole limpet hemocyanin (KLH) for immunization. Then H1b-KLH conjugation was injected into mouse subcutaneously to produce polyclonal antibody. ELISA assay was used to detect the titer of the antibody. Antibody was also identified by Western blot and immunohistochemistry assays. RESULTS: Mouse antibody against Hlb was prepared after injection of H1bKLH conjugation. The titer of H1b antibody was about 1:10~5.Western blot confirmed its high specificity. This antibody could also be used for immunohistochemistry analysis. CONCLUSION: The successful preparation of the polyclonal antibody against protein H1b, which can discriminate the two variants of the major subunit of ASGPR with high specificity, will provide an efficient reagent for further study of the physiologic functions of H1b and its role in the pathogenesis of human disease.
