Preliminary study on gene expression profiles of sporadic parathyroid adenoma with application of gene-chip technology

VernacularTitle:应用基因芯片技术初步探讨散发性甲状旁腺腺瘤基因表达谱
Author: Shunli GUO ; Jie KANG ; Youben FAN
Publication Type:Journal Article
Keywords: Parathyroid adenomas; Genes; Genechip
From: Journal of Endocrine Surgery 2009;3(3):154-157
CountryChina
Language:Chinese
Abstract: Objective To investigate the differentially expressed genes in sporadic parathyroid adenomas using genechip and provide basis for further studying the mechanism and diagnosis of sporadic parathyroid adeno-mas. Methods Total RNA was extracted from normal parathyroid tissues and adenomas tissues. The mRNA was used to prepare probes. The mixed probes were hybridized to the cDNA microarray. After washing, the DNA chips were scanned by laser scanner. The acquired image was analyzed. Results Among the 47 000 target genes, 2302 genes were identified in sporadic parathyroid adenomas tissues that had at least two-fold changes compared to normal parathyroid tissues, and 1012 genes up-regulated and 1290 down-regulated were found in this study. Of 10 genes up-regulated and 27 down-regulated, the changes were over five-fold. The function of these changed genes was involved in transport, cell adhesion, signal transduetion, transcription, ion binding and so on. Conclusions Many parathyroid adenomas associated genes have been screened by high-throughout gene chip method, Which may help to identify the mechanism and diagnosis of sporadic parathyroid adenomas.