A Study of the inhibitory effect of a recombinant CEA vaccinia virus inocnlation on mouse CEA positive tumor

VernacularTitle:接种 CEA重组痘苗病毒对小鼠 CEA＋肿瘤抑制作用的机制探讨
Author: Qing ZHAO ; Sha TAO ; Jie YANG ; Hongbing YAO ; Jinwei XIE ; Chaoquan LUO
Publication Type:Journal Article
From: Journal of Cellular and Molecular Immunology 2001;17(4):324-327
CountryChina
Language:Chinese
Abstract: Aim To explore antitumor mechanism of a recombinant vaccinia virus containing the human CEA-cDNA (rV-CEA). Methods C57/BL mice were immunized three times with rV-CEA. Six weeks later, the macrophages(MΦ s)and splenocytes from rV-CEA-immunized donors were transferred to CEA＋ -HePa tnmor-bearing recipients,Meanwhile, the antitumor effects of these donor's MΦ s and splenocytes and that of the recipient's splenocytes were detected in vitro. Results The MΦ s and splenocytes from rV-CEA-immunized donors possessed strong antitumor activity in CEA-positive tumor-bearing recipients. The in vitro antitumor effect of splenocytes from mice inoculated with MΦ s from rV-CEA-immunized donors were markedly stronger than those from W-VV-immunized donors. However,the in vitro antitumor effect of the MΦ s from rV-CEA-immunized donors was the same as those from W-VV-immunized donors. Conclusion It is demonstrated that antitumor activity induced with rV-CEA may be mediated mainly by antigen present cells (the MΦ s), which activated tumor-specific T cells to kill tumor cells.