The effect of arsenic sulfide combined with IFN-α on K562 cells
10.3321/j.issn:1007-8738.2009.10.020
- VernacularTitle:联合应用硫化砷和α干扰素对K562细胞的作用
- Author:
Jing LI
;
Shanxi LIU
;
Mei ZHANG
- Publication Type:Journal Article
- Keywords:
IFN-α;
arsenic sulfide;
apoptosis;
telomerase;
K562 cells
- From:
Journal of Cellular and Molecular Immunology
2009;25(10):929-931
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study if the effect of arsenic sulfide combined with IFN-α can be increased on K562 cells. METHODS: Telomerase activity was determined by PCRELISA. Flow cytometry was used to analyze the cell apoptosis. The final concentration of IFN-α and arsenic sulfide was 10 000 U/mL and 0.6 mg/L. RESULTS: The rates of apoptosis was 37.8% and 37% in K562 cells treated with IFN-α or arsenic sulfide alone for 8 days; The rates of apoptosis and inhibition of telomerase activity was 59.9% and 81.2% in K562 cells treated with IFN-α and arsenic sulfide simultaneously for 8 days, or 60.37% and 78.8% in K562 cells was treated with arsenic sulfide for 5 days after affected by IFN-α for 3 days. 71.3% telomerase activity was inhibited in K562 cells by arsenic sulfide alone for 8 days. CONCLUSION: Combination of arsenic sulfide and IFN-α can increase the apoptosis and inhibit the telomerase activity of K562 cells obviously comparing with the two drugs used alone. IFN-α maybe promote arsenic sulfide inducing apoptosis of K562 cells.
