Effects of non-wounded leg ischemic post-conditioning on the level of TNF-α and NO in serum of rats undergoing pancreas transplantation

VernacularTitle:无创伤双后肢缺血后处理对胰腺移植大鼠血清TNF-α和NO的影响
Author: Zhaohui ZHANG ; Weizhong WANG ; Xi LI ; Wancheng NIU ; Dangying CHEN ; Hongzun CUI ; Yunmin ZHANG
Publication Type:Journal Article
Keywords: Pancreas transplantation; Ischemic postconditioning; Non-wounded leg; TNF-α; NO; Rat
From: Journal of Endocrine Surgery 2011;05(1):18-20
CountryChina
Language:Chinese
Abstract: Objective To evaluate the effects of non-wounded leg ischemic post-conditioning on the serum TNF-α and NO level of rats undergoing pancreas transplantation. Methods Group Sham consisted of 6 normal SD rats. 12 diabetic SD rats were randomly assigned to 2 groups: I/R group consisted of 6 diabetic rats which received ischemia reperfusion and NWLIPO group consisted of 6 diabetic rats which received ischemic post-conditioning. Blood glucose was measured before and after reperfusion. The level of serum NO and TNF-α was monitored 2 hours after long-time reperfusion. Results The level of blood glucose and TNF-α in NWLIPO group was lower than that in I/R group (P＜0. 01) while the level of NO was higher in NWLIPO group than in I/R group (P＜0.01). Conclusion Non-wounded leg ischemic post-conditioning can increase serum NO synthesis and down-regulate TNF-α.