Effects of recombinant soluble MICA protein on the biologic activities of NK cells

VernacularTitle:重组可溶性MHC Ⅰ类相关蛋白A对NK细胞生物学活性的影响
Author: Weijuan GONG ; Haiyang WANG ; Minqi FAN ; Chunxiang GONG ; Dan LIU ; Mingchun JI
Publication Type:Journal Article
Keywords: MICA; NK cell; cytotoxicity; proliferation; apoptosis
From: Journal of Cellular and Molecular Immunology 2009;25(10):903-906
CountryChina
Language:Chinese
Abstract: AIM: To study the effects of recombinant soluble MHC class Ⅰ chain-related protein A (sMICA) on the cytotoxicity, secretion of IFN-γ, proliferation and apoptosis of peripheral NK cells. METHODS: After NK cells were cocultured with recombinant soluble MICA proteins overnight, the cytotoxicity of NK cell on target cells was detected by flow cytometry. The supemant was collected to determine the concentration of IFN-γ by ELISA. The proliferation of NK cells to sMICA was detected by MTS/PMS. NK cells were labeled with annexin V and PI to analyze their apoptosis. RESULTS: Soluble MICA inhibited the cytotoxicity of NK cells and down-regulated the secretion of IFN-γ, but it showed no effects on the proliferation and apoptosis of freshly isolated peripheral NK cells. CONCLUSION: The soluble MICA shedding from tumor cells could be a pathway of cancer immune evasion by down-regulating the biologic activities of NK cells.