Biological activity assays and cellular imaging of anti-human sperm protein 17 immunomagnetic nanoparticles
10.3321/j.issn:1007-8738.2009.11.007
- VernacularTitle:精子蛋白17抗体免疫磁性颗粒活性分析及细胞成像研究
- Author:
Qun LIU
;
Yuqing GE
;
Fangqiu LI
;
Shixin ZHANG
;
Ning GU
;
Zhongqiu WANG
;
Guangming LU
- Publication Type:Journal Article
- Keywords:
sperm protein 17 antibody;
immunomagnetic nanoparticles;
activity assays;
magnetic resonance imaging
- From:
Journal of Cellular and Molecular Immunology
2009;25(11):987-990
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To prepare anti-Sperm protein 17 (Sp17) immunomagnetic nanoparticles (IMNPs), and make foundation for target diagnosis of ovarian cancer by magnetic resonance imaging. METHODS: The anti-human Sp17 IMNPs were prepared by grafting anti-Sp17 antibodies on the surface of chitosan-coated magnetic nanoparticles （MNPs） using the linker of EDC/NHS (1-ethyl-3-［3-dimethylaminopropyl］carbodiimide/N-hydroxysuccinimide). The morphology and properties of the nanoparticles were characterized by transmission electronic microscopy (TEM), the conjugation of the antibodies was evaluated by native-polyacrylamide gel electrophoresis, the immunologic activity of IMNPs was evaluated by enzyme linked immunosorbent assay (ELISA). A set of in vitro magnetic resonance imaging (MRI) experiments were performed after incubated the IMNPs with human Sp17 gene transfected ovarian cancer HO-8910 cells. RESULTS: We had successfully grafted the MNPs with anti-Sp17 antibody and the IMNPs kept good bioactivity. The MRI showed that the IMNPs were targeted successfully to the positive cells, and no obviously non-specific adsorption was observed. CONCLUSION: The anti-Sp17 IMNPs with good specificity can used for further study of ovarian cancer target therapy.
