The correlation between MMP-2, MMP-13 and the axillary lymph node metastasis in breast cancer

VernacularTitle:MMP-2和MMP-13与乳腺癌腋窝淋巴结转移的相关性
Author: Xiaoli DENG ; Jianyi LI ; Wenhai ZHANG
Publication Type:Journal Article
Keywords: Breast cancer; Vascular thrombosis; MMPs; Lymph nodes metastasis
From: Journal of Endocrine Surgery 2011;05(6):365-369
CountryChina
Language:Chinese
Abstract: Objective To study the function of the important members of MMPs in breast cancer and their correlation with lymph nodes metastasis through making groups according to vascular thrombosis status showed by pathological diagnosis and detecting the expression of MMP-2,-9,-13,-14 in order.Methods Fluorescent quantitative RT-PCR was used to detect mRNA expression of MMP-2,-9,-13,-14 in 30 cases of breast cancer patients in both groups (negative and positive vascular thrombosis groups).Streptavidin-peroxidase methods (S-P) immunohistochemical method was employed to detect the expression of ER,PR,HER2,P53 and Ki-67 in breast cancer tissues.The data were analyzed by t test etc.Results Lymph nodes metastasis was significantly more in vascular thrombosis positive group than in negative group(P ＜ 0.05 ).MMP-2 and -13 were overexpressed in vascular thrombosis negative group (P ＜ 0.05).Conclusions Breast cancer patients with positive vascular thrombosis have a more apparent trend of lymph nodes metastasis.MMP-2 and MMP-13 mRNA play a negative regulatory role in intravasation of tumor cells by producing substances that may inhibit tumor angiogenesis and intravasation.