The purification of factor B in human sera by liquid chromatography
10.3321/j.issn:1007-8738.2001.01.031
- VernacularTitle:液相色谱法纯化人血清 B因子
- Author:
Bicheng ZHANG
;
Qing LI
;
Lian GUO
;
Yayu HUANG
- Publication Type:Journal Article
- From:
Journal of Cellular and Molecular Immunology
2001;17(1):82-83
- CountryChina
- Language:Chinese
-
Abstract:
Aim To establish a method to purify factor B in human sera. Methods A combination of euglobulin precipitation, ion-exchange chromatography,(NH4)2SO4 precipitation and affinity chromatography was used in the process of purification. Results Final product of 118.75 mg/L plasma factor B was obtained. By SDS-PAGE, thin layer scanner and activity assay,the purity reached 95％ , specific activity was 1.91× 109 IU/g, and the activity yield was 59.28％ . Conclusion This simple method with high yield can be used for laboratory research and large-scale preparation.
