study on the expression and biological activity of the expression products of the human β-NGF cDNA in COS-7 cell line
10.3321/j.issn:1007-8738.2001.04.004
- VernacularTitle:人β-NGF基因在 COS-7细胞中的表达及其生物学活性的研究
- Author:
Xiaofeng HAN
;
Lansheng ZHANG
;
Jingcheng MIAO
;
Jing ZHOU
;
Shoupeng ZHU
- Publication Type:Journal Article
- From:
Journal of Cellular and Molecular Immunology
2001;17(4):310-312
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the expression of β-NGF gene in the mammlian cells and the biological activity of its expression products. Methods The β-nerve growth factor (β-NGF) cDNA which obtained from the plasmid pGEM-β-NGF by enzyme digestion analysis was cloned into the expression vector pcDNA3 to construct the recombinant plasmid pcDNA3-β-NGF. COS-7-cell line grown to log phase was transfected using lipofectamine reagent. The expression level of β-NGF mRNA and the biological activity were analyzed by Northern blot and observation of neurite outgrowth of PC12 cell line stimulated by supernatant, respectively. Results The β-NGF gene was expressed successfully in COS-7 cell lines. The culture supernatant of the positive COS-7 cells transfected with pcDNA3-β-NGF could stimulate the neurite outgrowth of PC12 cell line. Conclusion The target gene expressed successfully in the transfected COS-7 cell line and had good biological activity.
