The protective effect on the co-transplanted allogeneic renal cells in male and female recipient rats by the testicular Sertoli cells expressing FasL
10.3321/j.issn:1007-8738.2001.04.017
- VernacularTitle:睾丸 Sertoli细胞对共移植同种异体肾细胞的保护作用及受体性别的影响
- Author:
Xiaoqing SUN
;
Hong ZHAO
;
Rumin WEN
;
Junnian ZHENG
;
Baoluo LIAN
- Publication Type:Journal Article
- From:
Journal of Cellular and Molecular Immunology
2001;17(4):350-352
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the protective effect of Sertoli cells on the co-transplanted allogeneic renal cells in male and female recipient rats. Methods Testicular Sertoli cells were prepared by digestion with trypsin， collagenase and DNase， while renal cells were prepared by digestion with trypsin alone. FasL and Fas expressions were detected respectively by FCM before transplantation. About 106 cells were injected into the allogeneic renal subcapsule. In order to demonstrate the survival of renal cells， the expression of g-PG in graft was examined by SABC method. Apoptosis of the lymphocytes surrounding the graft was observed by TUNEL(terminal deoxynucleotidyl transferase-mediated X-dUTP nick end labeling). Results The grafts were analyzed histologically 20 days after transplantation. The renal cells transplanted alone were all rejected, while the survival rates of the mixed cell transplantation were 87.5％ and 77.8％ in male and female recipients respectively. When the renal cells co-transplanted with Sertoli cells treated by anti-FasL mAb， only 30.0％ grafts were survived. Apoptosis of lymphocytes surrounding the graft were quite evident. Conclusion Sertoli cells expressing FasL can protect renal cells from allograft rejection by inducing apoptosis of Fas expressing T cells surrounding the grafts.
