Isolation and purification of canine islets
10.3760/cma.j.issn.1674-6090.2011.05.004
- VernacularTitle:犬胰岛的分离与纯化
- Author:
Jinfa FENG
;
Ping XU
;
Chun SONG
;
Yi ZHOU
- Publication Type:Journal Article
- Keywords:
Islet transplantation;
Isolation;
Purification;
Islet
- From:
Journal of Endocrine Surgery
2011;05(5):299-301
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate factors influencing islet isolation and purification in dogs and to explore how to obtain massive purified viable islets from canine pancreas.To provide experimental evidence for pancreatic islet transplantation in clinical practice.Methods Islets were isolated from pancreas of 22 dogs by modified automated techniques,followed by purification using continuous density gradients.The purity of islets was determined by dithizone (DTZ) staining,and the function of islets was evaluated by glucose stimulating insulin release test.Results The crude islets yield was ( 155 040 ±310) IEQ/pancreas while the yield was (74 200 ±185) IEQ/pancreas after purification.The average purity was (89.4 ±2.6) ％.The recovery rate was(47.8 ±1.3) ％.Islet viability was(93.0 ± 1.7)％.The modified techniques improved both the yield and the quality in islet isolation and purification.The islets were morphologically intact after purification.Glucose stimulating insulin release test showed that the difference of the secreted insulin concentration had statistical significance ( P ＜0.001 ) between low concentration glucose group and high concentration glucose group.Conclusion It is of great value for clinical research to obtain enough islets through islet isolation and purification in dogs.
