Protective effect of N-acetylcysteine on liver and lung in mice after ischemia-reperfusion injury
10.3321/j.issn:1007-8738.2009.11.032
- VernacularTitle:乙酰半胱氨酸对小鼠缺血再灌注损伤肝肺组织的保护作用及机制
- Author:
Shan ZENG
;
Yi LIN
;
Jingfang DI
;
Zheng FENG
- Publication Type:Journal Article
- Keywords:
N-acetylcysteine;
ischemia-reperfusion;
lung injury
- From:
Journal of Cellular and Molecular Immunology
2009;25(11):1058-1060
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate protective effect of N-acetylcysteine (NAC) on liver and lung in mice after hepatic ischemia/reperfusion injury. METHODS: BALB/c mice were used in a model of partial hepatic ischemia/reperfusion (I/R) injury.They are divided randomly to sham-operated control group(SH), hepatic I/R group or NAC pretreated in hepatic I/R group(I/R-NAC).The level of TNF-α in protal vein and plasma ALT were measured at 1hour and 3 hour, respectively after reperfusion.Lung tissue wet-to-dry(W/D) weight ratio compared. RESULTS: Lung tissue W/D ratio showed significant difference between two groups; The expressions of TLR2/4 mRNA in liver and lung increased obviously after hepatic I/R injury. Histological evaluation showed several changes in lung tissue in I/R group.The level of TNF-α and ALT in protal vein increased continually in I/R group at 1hour and 3 hour of reputation compared with SH group.The level of TNF-α and ALT declined significantly in the group pretreated by NAC. CONCLUSION: N-acetylcysteine can inhibit the activation of TLR2/4 and reduce TNF-α secretion resulted from I/R injury it might abate liver and lung injury following partial hepatic ischemia-reperfusion in mice.
