Expression of THANK in human PBMC activated with different stimulators

VernacularTitle:诱导活化的外周血单个核细胞 THANK表达的初步研究
Author: Dong WU ; Feng SHEN ; Yonghua LOU ; Binghua JIAO ; Mengchao WU
Publication Type:Journal Article
From: Journal of Cellular and Molecular Immunology 2001;17(4):304-306
CountryChina
Language:Chinese
Abstract: Aim To analyze THANK gene expression in peripheral blood mononuclear cells(PBMC) stimulated with different stimulators and to clone whole length human THANK gene. Methods PBMC were conventionally isolated and cultured in RPMI1640 containing 10％ FCS. After stimulated with LPS,TNF α ,IL 2,IFN γ ,PHA or PMA,the THANK gene expression in PBMCs was analyzed by RT PCR and THANK cDNA was cloned. Result RT PCR detection showed that THNAK gene expressed in PBMCs after stimulated with interferon γ for 3 days, whereas THANK'expression could not be detected after stimulated with LPS,TNF α ,IL 2,IFN γ ,PHA or PMA respectively. Then THANK gene was cloned by cloning PCR product and sequenced. Conclusion Human THANK gene is cloned successfully, thus providing the possibility for further research of THANK'function.