Purification and bioassay of a new type of human interferon α 1c

VernacularTitle:新型人干扰素α 1c的纯化和活性测定
Author: Jiang LI ; Wenliang WANG ; Kaikun WEI ; Xuejun MA ; Yunde HOU
Publication Type:Journal Article
From: Journal of Cellular and Molecular Immunology 2001;17(1):79-81
CountryChina
Language:Chinese
Abstract: Aim To express and purify a new sub-type of interferon α , α 1c in E.coli. Methods To establish a brief protocol for expression and purification of IFN-α 1c. In vitro assay for the IFN activity is required for the measurement of antiviral, antiproliferative and immunoregulatory properties. Results Expression of IFN-α 1c was verified by its reactivity to IFN-α 1-specific neutralizing antibodies by ELISA. Western blot also detected a band with relative molecular mass(Mr) of 19 000. IFN-α 1c possessed antiviral biological activity(3.2× 107) higher than IFN-α 1b(1.0× 107～ 1.8× 107) did on VSV challenged WISH cells. IFN-α 1c also inhibited growth rate of A549 comparable to IFN-α 1b. IFN-α 1c as an immunologic effector also augmented the cytotoxic activity of NK cells. Conclusion These results demonstrate that IFN-α 1c can be successfully expressed in E.coli. This interferon with high bioactivity may be a good candidate for clinical use.