Evaluation of a new flow cytometry based method for detection of BCR-ABL1 fusion protein in chronic myeloid leukemia.
- Author:
Swati DASGUPTA
1
;
Ujjal K RAY
;
Arpita Ghosh MITRA
;
Deboshree M BHATTACHARYYA
;
Ashis MUKHOPADHYAY
;
Priyabrata DAS
;
Sudeshna GANGOPADHYAY
;
Sudip ROY
;
Soma MUKHOPADHYAY
Author Information
- Publication Type:Comparative Study ; Original Article
- Keywords: Chronic myeloid leukemia; BCR-ABL1; Philadelphia chromosome; Flow cytometry
- MeSH: Arm; Cytogenetics; Diagnosis; Flow Cytometry*; Fusion Proteins, bcr-abl; Humans; Karyotyping; Leukemia, Myelogenous, Chronic, BCR-ABL Positive*; Methods*; Philadelphia Chromosome; Real-Time Polymerase Chain Reaction
- From:Blood Research 2017;52(2):112-118
- CountryRepublic of Korea
- Language:English
- Abstract: BACKGROUND: Philadelphia chromosome, a hallmark of chronic myeloid leukemia (CML), plays a key role in disease pathogenesis. It reflects a balanced reciprocal translocation between long arms of chromosomes 9 and 22 involving BCR and ABL1 genes, respectively. An accurate and reliable detection of BCR-ABL fusion gene is necessary for the diagnosis and monitoring of CML. Previously, many technologies, most of which are laborious and time consuming, have been developed to detect BCR-ABL chimeric gene or chromosome. METHODS: A new flow cytometric immunobead assay was used for detection of BCR-ABL fusion proteins and applicability, sensitivity, reliability, efficacy and rapidity of this method was evaluated. RESULTS: From February 2009 to January 2014, a total 648 CML patients were investigated for the status of BCR-ABL1 protein. Among them, 83 patients were enrolled for comparative study of BCR-ABL1 positivity by three routinely used procedures like karyotyping, and quantitative real time PCR (RT-PCR) as well as immunobead flow cytometry assay. BCR-ABL protein analysis was found consistent, more sensitive (17% greater sensitivity) and reliable than the conventional cytogenetics, as flow cytometry showed 95% concordance rate to RT-PCR. CONCLUSION: BCR-ABL fusion protein assay using a new flow cytometric immunobead might be useful in the diagnosis and monitoring CML patients.
