Cloning and expression of the 1.2kb cDNA fragment of human integrin α 4 subunit

VernacularTitle:人整合素分子α 4亚基片段的克隆和表达
Author: Yongquan LIU ; Jieying GAO ; Zhenge LUO ; Xiangying KONG ; Junjie MEI ; Hong PENG
Publication Type:Journal Article
From: Journal of Cellular and Molecular Immunology 2001;17(1):13-15
CountryChina
Language:Chinese
Abstract: Aim To clone and express the 1.2kb cDNA fragment (1/753-2 934bp) of human integrin α 4 subunit. Methods The 1.2 kb cDNA fragment of human integrin α 4 subunit was amplified from HL-60 total RNA by RT-PCR, then it was subcloned into expression vector pGEX-3X and induced with IPTG. Results The 1.2 kb cDNA fragment of human integrin α 4 subunit was cloned. The sequencing indicated that there was only one missense mutation (Arg→ Gln) among the fragment, and this mutation won't affect antigenicity after analysed by GOLDKEY. Then the 1.2 kb cDNA was subcloned into expression vector pGEX-3X. The α 4 fragment was highexpressed in E.coli after induced with IPTG. Conclusion The 1.2kb cDNA fragment of α 4 subunit was obtained, and it was highexpressed in E.coli, it might be important for study on the function of α 4 integrins.