The effects of adipose-derived stem cell transplantation on the expression of IL-10 and TNF-α after cerebral ischaemia in rats
10.3321/j.issn:1007-8738.2009.11.010
- VernacularTitle:脂肪来源的干细胞移植对脑缺血大鼠脑组织IL-10及TNF-α表达的影响
- Author:
Houwei DU
;
Nan LIU
;
Jiehua WANG
;
Yixian ZHANG
;
Ronghua CHEN
;
Yingchun XIAO
- Publication Type:Journal Article
- Keywords:
adipose-derived stem cell;
cerebral ischemia;
IL-10;
TNF-α
- From:
Journal of Cellular and Molecular Immunology
2009;25(11):998-1001
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore the effects of adipose-derived stem cell (ADSC) transplantation on the expression of IL-10 amd TNF-α after cerebral ischaemia in Middle cerebral artery occlusion (MCAO) rats. METHODS: 72 male adult Sprague-Dawley rats were randomly divided into 4 groups: sham group, MCAO group, Vehicle group and ADSC group (n=18). Rat's cerebral ischemia model was established by MCAO with Longa' s method. ADSC were labeled by DAPI before transplantation. One day after MCAO, 30 μL of cell suspension containing 1×10~6 ADSCs were injected into the lateral ventricle of ADSC group and the same dose of PBS was given to the Vehicle group. At day 4, day 7 and day 14 after MCAO, the rats were decapitated to detect the expression of IL-10 and TNF-α in ischaemic rat' s brain by ELISA, immunohistochemistry and RT-PCR. RESULTS: Compared with sham group, the expression of IL-10 and TNF-α significantly up-regulated at 4 d, 7 d of MCAO group(P<0.05). There was no statistical difference of IL-10 and TNF-α expression between MCAO and vehicle group ant any time point(P>0.05). Compared with Vehicle group, the expression of IL-10 significantly up-regulated while TNF-α expression significantly decreased of ADSC-treated group at any timepoint post MCAO(P<0.05). CONCLUSION: The transplantation of ADSC could up-regulate the expression of IL-10 and down-regulate the expression of TNF-α in MCAO rat' s brain, which might contribute to its protective role upon cerebral ischaemia.
