Effect of high intensity ultrasound on COX-2 mRNA expression in human breast cancer cells
10.3760/cma.j.issn.1674-6090.2011.06.004
- VernacularTitle:高强度超声对人乳腺癌细胞中COX-2mRNA表达的影响
- Author:
Jiangtao HAN
;
Guangyun SUN
;
Jianpu HUANG
;
Lei TANG
;
Jirong LIU
;
Mingjun XIE
- Publication Type:Journal Article
- Keywords:
High intensity ultrasound;
COX-2 mRNA;
Breast cancer;
Apoptosis
- From:
Journal of Endocrine Surgery
2011;05(6):374-376
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the mechanism of high intensity ultrasound (HIU) on COX-2 mRNA expression and apoptosis in breast cancer cell line MDA-MB-231 cells.Methods Human breast cancer cells MDA-MB-231 in vitro were exposed in HIU (50 w/cm2).RT-PCR was performed to detect the level of COX-2 mRNA before and after exposure.The ultrastructural changes in apoptotic cells were examined by transmission electron microscopy.Results The relative level of COX-2 mRNA decreased gradually along with the increase of exposure time and apoptotic bodies in MDA-MB-231 cells considerably increased under electron microscope.When exposure time was increased to 30 seconds,a few cells died.Conclusion HIU promotes apoptosis of breast cancer MDA-MB-231 cells in a COX-2 mRNA depended way.
