An experimental study on inhibiting growth and metastasis of mouse melanoma by engineering endostatin
10.3321/j.issn:1007-8738.2001.01.023
- VernacularTitle:重组人血管内皮抑制素抗肿瘤效应的应用研究
- Author:
Jiangqiu LIU
;
Zhongyi LI
;
Linsheng CHEN
;
Yihong SUN
;
Lu XU
;
Junyuan WANG
;
Wanxing LIU
;
Jielai XIA
- Publication Type:Journal Article
- From:
Journal of Cellular and Molecular Immunology
2001;17(1):63-64
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore inhibitory effects and mechanism of engineering endostatin on growth and metastasis of melanoma cells in mice. Methods Melanoma cells(2× 106/mouse)were inoculated sabcutaneously to C57BL/6 mice. After tumorigenesis,endostatin(8mg/kg.d)was administrated to tumor-bearing mice,once a day ,twenty-one in all.Dietetic state and weight change of the tumor-bearing mice were observed and tumorous sige was measured during administration of endostatin. On 26thday,the tumor-bearing mice were sacrificed,subcutaneously tumorous weight was weighed and brain,lung ,liver,spleen and kidney were excised and sections were made to supply the pathological examination. Results Area under curve in the endostatin-treated group was obviously less than that in tamor control group(P∨ 0.01). Pathological study revealed that lavge areal necrosis arose in tumor and newborn cappillaries around the tumor disapeared. Conclusion Endostatin possosses strongly inhibitory effects on growth and metastasis of mouse melanoma and formation of newborn capillaries around tumor.
