The study of changes on NKT cells of experimental autoimmune encephalomyelitis (EAE) mice

VernacularTitle:实验性自身免疫性脑脊髓炎小鼠脾脏和肝脏中NKT细胞调变的研究
Author: Qing OUYANG ; Kun CHEN ; Xi WANG ; Chunmei ZHANG ; Jun GUO ; Yuying WEI ; Yuanjie SUN ; Zhuwei XU ; Kun YANG
Publication Type:Journal Article
Keywords: NKT cell; EAE; immunoregulation
From: Journal of Cellular and Molecular Immunology 2009;25(10):894-896
CountryChina
Language:Chinese
Abstract: AIM: To observe the changes of the number of NKT cells in spleens and livers of induced model of experimental autoimmune encephalomyelitis (EAE), and to study the role NKT cells play in the immunoregulation of EAE. METHODS: C57BL/6 mice were immunized with MOG<,35-55> peptide and received clinical evaluation daily. The mice were sacrificed at the fastigium and the splenic and hepatic lymphocytes were isolated. The changes of NKT cells in normal and EAE C57BL/6 mice were detected by flow cytometry. RESULTS: The percent of NKT cells in lymphocytes of different organs of EAE model were greater decreased than in that of normal mice. The percent of NKT cells in splenic lymphocytes of normal mice was 2.22± 0.14, while that in EAE mice was 1.94±0.07 (P < 0.05). The percent of NKI cells in hepatic lymphocytes of normal mice was 5.52±2.17, while that in EAE mice was 2.67± 1.41 (P < 0.05). CONCLUSION: The proliferation of splenic and hepatic NKT cells in C57BL/6 mice are inhibited in EAE model, which may indicate that the immune function conducted by NKT cell is down regulated in EAE mice.