Screening antigenic epitopes from HCV core protein random peptide libraries displayed on phage

VernacularTitle:从 HCV核心蛋白噬菌体随机展示肽库中筛选抗原表位
Author: Wei PAN ; Zhongtian QI ; Xiaolan WU ; Xin PAN ; Qiuli CHEN ; Ping DU ; Xiang HE
Publication Type:Journal Article
From: Journal of Cellular and Molecular Immunology 2001;17(1):20-23
CountryChina
Language:Chinese
Abstract: Aim To screen out the antigenic sequences from HCV core protein random peptide libraries displayed on phage and to explore a new way to screen the viral antigens. Methods The anti-HCV core antibody-positive serum was used to screen antigenic peptides from the HCV core protein random peptide libraries displayed on phage for 4 rounds. Detection of numbers of positive clones, positive rate of insertion of HCV random DNA and positive rate of hybridization with HCV core probes were used to evaluate the screening effects. The DNA sequences of 7 selected clones with positive hybridization were determined and analysed. Results Six out of 7 sequences are HCV core protein sequences, in which 5 were perfectly displayed,and one was possibly displayed. These sequences included several major HCV core antigenic epitopes. The remaining one was E.coli nrfa gene. Conclusion The phage display technique can be applied to study the viral antigenic peptides with the advantages of simple, accuracy and rapidity.