Screening of mimicry peptide of Japanese encephalitis virus E protein from phage 15-mer peptide library
10.3321/j.issn:1007-8738.2001.04.011
- VernacularTitle:从噬菌体 15肽库中筛选乙脑病毒糖蛋白模拟肽
- Author:
Junping REN
;
Wenyu MA
;
Qiaoxin YANG
;
Tianbing DING
;
Yi XIAO
;
Jianhua SONG
- Publication Type:Journal Article
- From:
Journal of Cellular and Molecular Immunology
2001;17(4):332-334
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the mimicry peptide of JEV E protein by screening a phage 15-mer peptide library with anti-JEV E protein mAb 2H4. Methods After three rounds biopanning, the enriched positive phage clones were identified by ELISA. 10 positive phage clones were sequenced and compared homologously with JEV E protein. Results The short peptide displayed on screened positive phage could bind specifically to mAb 2H4, and the binding could be inhibited by natural JEV Ag. Amino acid sequences of the 10 positive phage clones were consensus, that is, RQDPQWPYANSTIAR. By homology analysis, two higher homologous sequences STXAR and WXXAXST were found in different regions of JEV E protein. The peptide displayed on positive phage could react specifically with the mouse antiserum against natural JEV Ag . Conclusion This peptide displayed on positive phage may mimic partial antigenicity of JEV E protein.
