Preparation and characterization of a novel functional anti-human CD83 monoclonal antibody

VernacularTitle:一株识别CD83单克隆抗体的研制及其生物学特性鉴定
Author: Chao GAO ; Weixue ZHONG ; Shudan ZHENG ; Liwen CHEN ; Yibei ZHU ; Xueguang ZHANG
Publication Type:Journal Article
Keywords: CD83; hybridoma; monoclonal antibody
From: Journal of Cellular and Molecular Immunology 2009;25(10):914-916
CountryChina
Language:Chinese
Abstract: AIM: To prepare and characterize a novel anti-human CD83 monoclonal mAb. METHODS: Female BALB/c mice of 6-8 weeks old were immunized with CD83 transfectant (L929/CD83) as immunogen. The spleen B cells of the mice were fused with Sp2/0 myeloma cells. The hybridoma cells were screened with CD83 transfectant (L929/CD83 and 293/CD83) by FCM. The biological characteristics of antibody were investigated by rapid isotyping analysis, karyotype analysis, competitive inhibition test and Western blot. RESULTS: One hybridoma cell line named 9D8 was obtained, which had the property of secreting antihuman CD83 monoclonal antibody steadily, This mAb specifically recognized CD83 molecule expressed on human mature DC, activated T cells, and tumor cell line Daudi, myeloma cell line 8226. This mAb can recognize a distinct epitope from comercial mAb(HB15e). CONCLUSION: One hybndoma cell line has been developed successfully, which may lay a fundation for further study on the function of this molecule.