Expression and characterization of fusion pro-tein hTNFα -hPgnK5 containing thrombin cleav-age site in E. coli

VernacularTitle:含凝血酶切点的 hTNFα -hPgnK5的原核表达及活性鉴定
Author: Bin FAN ; Yingqi ZHANG ; Ning ZHAO ; Zhen YAN ; Libo YAO ; Chengzhi SU
Publication Type:Journal Article
From: Journal of Cellular and Molecular Immunology 2001;17(1):29-31
CountryChina
Language:Chinese
Abstract: Aim To express the fusion protein hTNFα -hPgnK5 in E.Coli and to identify its activity. Methods The hTNFα -hPgnK5 gene express vector was constructed and the fusion protein was expressed in E.coli. The activity of fusion protein to inhibit the proliferation of tumor cells or angioendothelial cells in vitro was determined by MTT. Results Fusion protein hTNFα -hPgnK5 possessed immunoactivity in Western blot analysis and could inhibit proliferation of tumor cells or angioendothelial cells in vitro test. Conclusion The hTNFα gene and hPgnk5 gene can be coexpressed in prokaryotic system and expression products can inhibit the proliferation of tumor cells or angioendothelial cells in vitro.