Screening and identification of anti-HRPII single chain Fv antibodies of Plasmodium falciparum
10.3321/j.issn:1007-8738.2000.03.001
- VernacularTitle:抗恶性疟HRP-11单链抗体的筛选和鉴定
- Author:
Weiwen XU
;
Wenqi DONG
;
Ming LI
;
Huixiang BI
;
Baihong CHEN
;
Ping WANG
- Publication Type:Journal Article
- Keywords:
phage-display antibody library;
single-chain Fv antibody;
histidine-rich protein-II
- From:
Journal of Cellular and Molecular Immunology
2000;16(3):185-188
- CountryChina
- Language:Chinese
-
Abstract:
To develop simple, rapid, and efficacious diagnostic methods for malaria is one of the remaining key tasks for malaria control. Previously, we have created a phage-displayed antibody library against Plasmodium falciparum. Six clones of antibody with good reactivity to HRP-II in ELISA were isolated from the library after 3 rounds of enrichment. Soluble ScFvs were produced and the characteristics were determined. The results of Western blot showed that they could bind to HRP-II specifically and had a relative molecular mass(Mr) about 31 000. The work provided a solid fund for diagnostic kit development for malaria.
