Impacts of specific TDGF-1 gene silience by siRNA on invasion and migration of human breast cancer cell
10.3760/cma.j.issn.1674-6090.2010.05.002
- VernacularTitle:特异性siRNA沉默TDGF-1基因表达对乳腺癌细胞迁移侵袭的影响
- Author:
Pengcheng JIANG
;
Yu FAN
;
Yongjing ZHOU
;
Zhengyan WU
- Publication Type:Journal Article
- Keywords:
Breast carcinoma;
Teratocarcinoma-derived growth factor-1;
Invasion;
Migration;
Small interfering RNA
- From:
Journal of Endocrine Surgery
2010;04(5):291-294
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the impact of TDGF-1 gene silience by small interfering RNA(siRNA)on the invasion and migration of human breast cancer cell. Methods 3 siRNA fragments were designed according to the characteristic of TDGF-1 gene sequence and the most appropriate siRNA was selected by fluorescence real-time quantitative RT-PCR method. After the human breast cancer cell line MDA-MB-468 was transfected by the selected TDGF-1 siRNA, mRNA and protein of TDGF-1 were determined by real time quantitative RT-PCR and western blot respectively. The migration and invasion ability of the cancer cell were evaluated by wound-healing assay and Boyden chamber model respectively. Results siRNA could down-regulate the level of mRNA and protein of TDGF-1 in a dose-and time-dependent manner. In vitro experiment showed that TDGF-1 siRNA transfection can effectively inhibit the clonal growth, invasion and migration of breast cancer cell in a dose-dependent manner. Conclusions TDGF-1 gene may play an important role in the migration and invasion of human breast cancer cells. siRNA transfection can inhibit the invasion of human breast cancer cells.
