IL-6 selectively activates STAT3 in U937 cells
10.3321/j.issn:1007-8738.2001.01.005
- VernacularTitle:IL-6在U937细胞中特异、持续激活 STAT3
- Author:
Jiyan ZHANG
;
Yan LI
;
Beifen SHEN
- Publication Type:Journal Article
- From:
Journal of Cellular and Molecular Immunology
2001;17(1):16-19
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the signaling basis of the biological effects of IL-6 in U937 cells. Methods We determined the effect of IL-6 on the growth and differentiation of U937 cells and the effect of IL-6 on the activation of nuclear factors in U937 cells. Results IL-6 induced macrophage differentiation of U937 cells. Differentiated cells had strengthened ANAE (acid naphthyl acetate esterase)and NBT(nitroblue tetrazolium)-reducing activities and CD54 expression was upregulated. STAT3 could be remarkably activated by IL-6 in U937 cells and STAT3 activity was dependent on dose and time course of IL-6 treatment. However, NF-IL6,NF-κ B, AP-1 and other STAT members were not activated by IL-6. Conclusion IL-6 might induce terminal differentiation of U937 cells via JAK-STAT3 pathway.
