Biological effects of lipopolysaccharide, transforming growth factor-β1 on murine bone marrow-derived dendritic cells
10.3321/j.issn:1007-8738.2009.10.008
- VernacularTitle:脂多糖、转化生长因子-β1对小鼠骨髓来源树突状细胞的生物学作用
- Author:
Linlin FENG
;
Jihong DAI
;
Zhou FU
;
Zheng LIU
;
Lijia WANG
;
Xin LI
- Publication Type:Journal Article
- Keywords:
lipopolysaccharide;
transforming growth factor-β1;
mouse;
dendritic cells;
biological character
- From:
Journal of Cellular and Molecular Immunology
2009;25(10):891-893
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore method of stimulating murine bone marrow-derived dendritic cells by lipopolysaccharide(LPS), transforming growth factor-β1 (TGF-β1)and to study their biological character. METHODS: Murine bone marrow-derived dendritic cells were cultivated with cytokine GM-CSF and IL-4 for 6 days, BMDC was stimulated by control, LPS, TGF-β1, LPS +TGF-β1 for 48 hours respectively. Morphological characters of BMDC were observed by a inversed microscope, surface molecules such as CD_(11C), CD_(80), CD_(86)and MHC Ⅱ were detected by flowcytometry, Interleukin-6 and interleukin-12 p70 in co-culture medium was quantified by ELISA. RESULTS: In LPS group it presented the most typical DC morphology with the highest expression of CD_(80), CD_(86) and MHC Ⅱ, the strongest ability in mixed lymphocyte reaction, higher level of IL-6 and IL-12 p70 compared with control, TGF-β1, LPS + TGF-β1 ( P < 0. 05). While in TGF-β1 group it presented the less typical DC morphology with the lower expression of CD_(80), CD_(86), MHC Ⅱ, weaker ability in mixed lymphocyte reaction, and lower levels of IL-6 and IL-12 p70 compared with control and LPS (P < 0.05). CONCLUSION: LPS can stimulate maturation of BMDC in its late differentiation which makes it presents a more significant biological characteristics. TGF-β1 can inhibit maturation but not differentiation of BMDC thereby can prevent its biological characteristic presentation.
