Effect of soluble endothelial protein C receptor on natural killer cells and Th17 cells in patients with epithelial ovarian cancer
10.3760/cma.j.issn.1674-6090.2015.01.017
- VernacularTitle:可溶性血管内皮细胞蛋白C受体对上皮性卵巢癌患者自然杀伤细胞和Th17细胞的影响
- Author:
Furong CHENG
;
Min HU
;
Qing WANG
;
Zhengqiang WU
;
Jiangping SHI
;
Xiaomei LIU
- Publication Type:Journal Article
- Keywords:
sEPCR;
Epithelial ovarian cancer;
NK cells;
Th17 cells
- From:
Journal of Endocrine Surgery
2015;9(1):60-62
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of soluble endothclial protein C receptor(sEPCR) on natural killer(NK) cells and Th17 cells of epithelial ovarian cancer(EOC) patients and further to study the mechanism of the occurrence and development of epithelial ovarian cancer.Methods 35 cases with epithelial ovarian cancer were selected as the experimental group,and 35 healthy women were chosen as the control group.Peripheral venous blood sample(8 ml)was extracted from each subject.Plasma,serum and peripheral blood mononuclear cells(PBMCs) were obtained from the blood sample.ELISA was used to detect the level of plasma sEPCR and serum IL-17 and IL-21.Flow cytometry was used to detect proportions of NK cells and Th17 cells in PBMCs.Results Compared to the control group,the level of plasma sEPCR in the experimental group increased significantly(P ＜ 0.05).The level of serum IL-17,IL-21 and proportion of NK and Th17 cells in PBMCs significantly decreased(P ＜ 0.05).Correlation analysis showed that sEPCR was negatively correlated with IL-17,IL-21,Th17 cells and NK cells,and the correlation coefficient r was-0.71,-0.62,-0.68 and-0.79,respectively.Conclusion sEPCR in epithelial ovarian cancer patients possibly promotes the proliferation of tumor cells through inhibition of NK and Th17 cells.
