Freeze-Dried Human Platelet-Rich Plasma Retains Activation and Growth Factor Expression after an Eight-Week Preservation Period.
10.4184/asj.2017.11.3.329
- Author:
Yasuhiro SHIGA
1
;
Go KUBOTA
;
Sumihisa ORITA
;
Kazuhide INAGE
;
Hiroto KAMODA
;
Masaomi YAMASHITA
;
Toru ISEKI
;
Michihiro ITO
;
Kazuyo YAMAUCHI
;
Yawara EGUCHI
;
Takeshi SAINOH
;
Jun SATO
;
Kazuki FUJIMOTO
;
Koki ABE
;
Hirohito KANAMOTO
;
Masahiro INOUE
;
Hideyuki KINOSHITA
;
Takeo FURUYA
;
Masao KODA
;
Yasuchika AOKI
;
Tomoaki TOYONE
;
Kazuhisa TAKAHASHI
;
Seiji OHTORI
Author Information
1. Department of Orthopaedic Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan. yshiga1111@yahoo.co.jp
- Publication Type:Original Article
- Keywords:
Platelet-rich plasma;
Freeze drying;
Blood preservation;
Growth factors
- MeSH:
Blood Preservation;
Flow Cytometry;
Freeze Drying;
Healthy Volunteers;
Humans*;
Intercellular Signaling Peptides and Proteins;
Pathology;
Platelet Activation;
Platelet Count;
Platelet-Rich Plasma*;
Regeneration
- From:Asian Spine Journal
2017;11(3):329-336
- CountryRepublic of Korea
- Language:English
-
Abstract:
STUDY DESIGN: Controlled laboratory study. PURPOSE: This study aimed to evaluate the efficacy of platelet-rich plasma (PRP) stored at room temperature (RT), frozen, or after freeze-drying. OVERVIEW OF LITERATURE: PRP enriches tissue repair and regeneration, and is a novel treatment option for musculoskeletal pathologies. However, whether biological activity is preserved during PRP storage remains uncertain. METHODS: PRP was prepared from blood of 12 healthy human volunteers (200 mL/person) and stored using three methods: PRP was stored at RT with shaking, PRP was frozen and stored at −80℃, or PRP was freeze-dried and stored at RT. Platelet counts and growth factor content were examined immediately after preparation, as well as 2, 4, and 8 weeks after storage. Platelet activation rate was quantified by flow cytometry. RESULTS: Platelet counts were impossible to determine in many RT samples after 2 weeks, but they remained at constant levels in frozen and freeze-dried samples, even after 8 weeks of storage. Flow cytometry showed approximately 80% activation of the platelets regardless of storage conditions. Almost no growth factors were detected in the RT samples after 8 weeks, while low but significant expression was detected in the frozen and freeze-dried PRP. Over time, the mean relative concentrations of various growth factors decreased significantly or disappeared in the RT group. In the frozen group, levels were maintained for 4 weeks, but decreased significantly by 8 weeks (p <0.05). The freeze-dried group maintained baseline levels of growth factors for the entire 8-week duration. CONCLUSIONS: Freeze-drying enables PRP storage while maintaining bioactivity and efficacy for extended periods.
