RNAlater in parathyroid adenoma tissue storage

VernacularTitle:RNAlater在保存甲状旁腺腺瘤中的研究和探索
Author: Jianguo ZHAO ; Zhao JIN ; Ya HU ; Quan LIAO ; Yupei ZHAO
Publication Type:Journal Article
Keywords: Parathyroid adenoma; RNAlater; Degradation; β-actin
From: Journal of Endocrine Surgery 2014;8(1):29-32
CountryChina
Language:Chinese
Abstract: Objective To investigate different methods in parathyroid adenoma tissue storage and to provide theoretical support for standardized construction of parathyroid adenoma tissue data.Methods 22 samples of sporadic parathyroid adenoma in Beijing Union Hospital from Aug.2010 to Nov.2011 were collected.All cases obtained pathological diagnosis,among which 11 were kept in frozen nitrogen (group A)and the rest in RNAlater(group B).The ratio of OD 260/280 was measured by ultraviolet spectrophotometer.The integrity of RNA was examined by gel electrophoresis.The expression of β-actin was measured by Realtime-PCR technique.Results RNAlater group had no advantage than frozen nitrogen group in terms of the purity,concentration,and integrity of the extracted RNA(P ＞0.05),but had a higher expression level of the β-actin(P ＜0.05).Conclusion The method of RNAlater has no significant advantages compared with frozen nitrogen in terms of RNA prevention and RNA degradation,but is more effective in longer storage of samples.