Influence of MTDH gene down regulation on proliferation and apoptosis of human breast cancer SK-BR 3 cells
10.3760/cma.j.issn.1674-6090.2013.05.003
- VernacularTitle:转移粘附基因下调对乳腺癌SK-BR-3细胞增殖和凋亡的影响
- Author:
Cheng DU
;
Zhaozhe LIU
;
Zhenyu DING
;
Fang GUO
;
Dongchu MA
;
Xiaodong XIE
- Publication Type:Journal Article
- Keywords:
MTDH;
siRNA;
Breast cancer;
Proliferation;
Cell cycle;
Apoptosis
- From:Journal of Endocrine Surgery
2013;7(5):359-363
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate if down regulation of MTDH could inhibit proliferation and induce apoptosis in breast cancer SK-BR-3 cells.Methods RNA interference was employed to reduce MTDH expression in human breast cancer SK-BR-3 cells.Western blot assay was applied to measure the down regulation of MTDH.MTT assay was performed to assess the proliferation of SK-BR-3 cell.Flow cytometry was employed to detect cell cycle and apoptosis.Western blot assay was applied to detect the molecular alterations that was associated with cell proliferation,cell cycle and apoptosis.Results MTDH down regulation significantly inhibited cell proliferation.48 hours and 72 hours after trasnfection,the absorbance value(A value)in blank control,negative control and treatment group was (2.0 ± 0.1) vs (1.9 ± 0.3) vs (0.9 ± 0.1) (P =0.02) and (2.7 ± 0.2) vs (2.5 ± 0.4) vs (1.3 ± 0.2) (P =0.008).MTDH down regulation resulted in accumulation of the G0/G1 phase cells and reduction of S and G2/M phase cells.Moreover,MTDH down regulation significantly induced cell apoptosis.The cell apoptosis rate in blank control,negative control and trial group was (1.3 ± 0.2) ％,(1.4 ± 0.3) ％ and (19.6 ± 2.7) ％ (P =0.012).MTDH down regulation resulted in a decreased expression of cyclinD1 and Bcl-2,an increased expression of P21 and the activation of caspase-3.Conclusions Reduced MTDH expression in SKBR-3 cells can inhibit proliferation and induce apoptosis,which may be associated with decreased expression of cyclinD1 and Bcl-2,an increased expression of P21 and the activation of caspase-3.
